Biodiversity Genomics Europe Workshop

BRIGHT - Biodiversity Research Integrating Barcoding, Genomics and High-throughput Technologies
Biodiversity Genomics Europe (BGE) Joint Network Training Workshop
Dates: 3-7 February 2025

Targeted audience: Polish students, young researchers, citizen scientists, stakeholders.

Knowledge requirement: Basic knowledge of biology, especially knowledge and interest in biodiversity, nucleic acids composition and function.

Costs: the course is free of charge (including coffee breaks); travel, accommodation and lunches are not covered

Language: English

Contact: polbol@biol.uni.lodz.pl

Form of participation:

1.       Practical + theoretical, in person (up to 20 participants)

2.      Theoretical only, online (up to 40 participants)

20 places are secured for in person participation in the course. The choice will be made based on your answers  in the registration form. The priority will be given to students, early carrier researchers, stake holders, citizen scientists.

Address: Banacha 12/16, 90-237 Łódź. Faculty of Biology and Environmental Protection, Department of Invertebrate Zoology and Hydrobiology, University of Lodz

Organizers: PolBOL UniLodz (polbol.uni.lodz.pl) and Biodiversity Genomics Europe consortium (biodiversitygenomics.eu)

Registration deadline: 18 December 2024.

Results of evaluation: 20 December 2024.

Thanks to the synergy of DNA barcoding, genomics methods and high-throughput sequencing technologies, recognition of biodiversity from individuals through populations to species levels became available for everyone. The workshop will provide practical training and theoretical knowledge on using modern molecular tools in a wide aspect of biodiversity studies.

Workshop covers (theoretical and practical):

  • Material sampling and storage

  • DNA isolation (barcoding and genomics)

  • DNA barcoding (amplification, quality control, sequencing with Oxford Nanopore Techniques)

  • Sequencing technologies and their applications

  • Genomic data applications and databases (reference genomes, conservation genomics)

  • Data analysis and management (databases: BOLD, ENA; bioinformatic pipelines)

  • Citizen Science

 

Material for the course:

During the course we will focus on model invertebrate group - pollinators collected from Impatiens spp. from different sampling sites in Poland.

Genome: Gammarus sp. collected around the city of Lodz.


BGE training general schedule:

 

Monday (03.02)

Tuesday (04.02)

Wednesday (05.02)

Thursday (06.02)

Friday (07.02)

9.00 -10.30

Welcome, morning coffee

Introduction of attendees and instructors

DNA isolation for barcoding (Lab)

DNA amplification for Nanopore sequencing,

QC of HMW DNA 

Large-scale barcoding using Nanopore sequencing (AS)

Practical on genome assembly using Galaxy (practical on PC, TB)

10.45-12.00

DNA barcoding and BGE  (MG)

BOLD database, Sequence ID (TR)

ONTbarcoder

and  sequence ID (AS)

12.00

lunch break

lunch break

lunch break

lunch break

lunch break

13.15-14.30

Planing DNA-based biodiversity study (PG)

Collecting material in practice (PG, GK)

DNA barcoding of fungi (IS)

Museomics 101: How to harvest historical DNA from zoological collections? (MK)

visit in Biobank.

Overview of Illumina sequencing (MS)

14.45-16.30

eDNA in biodiversity assessment (KBS, AD)

High Molecular Weight (HMW) DNA Isolation 

DNA pooling,

ONT library preparation,

QC

FAIR data management, ENA (JP)

Practical on genome assembly using Galaxy (practical on PC, TB)

16.45-18.00

Molecular lab class 0

DNA Isolation methods (TM)

DNA amplification and quality control (TM)

Reference genomes/Galaxy (TB)

Citizen Science for biodiversity research(KBS)

Summary of the course

 

Lectures

Practical classes in wet laboratory

Practical classes outside or using computers

Instructors:

Lecturers:

Michał Grabowski (MG)

Piotr Gadawski (PG)

Grzegorz Tończyk (GT)

Tomasz Mamos (TM)

Tomasz Rewicz (TR)

Karolina Bącela-Spychalska (KBS)

Thomas Brown (TB)

Amrita Srivathsan (AS)

Igor Siedlecki (IS)

Andrea Desiderato (AD)

Marcin Kamiński (MK)

Joana Pauperio (JP)

Marta Sobalska (MS)

Laboratory instructors (Lab):

Tomasz Mamos (TM)

Gabriela Karlik (GK)

Tomasz Rewicz (TR)

Łukasz Trębicki (ŁT)

Wiktoria Cechowicz (WC)

Karolina Biniek (KB)

Piotr Gadawski (PG)

Serena Mucciolo (SM)

Computing instructors:

Amrita Srivathsan (AS)

Thomas Brown (TB)

Tomasz Mamos (TM)

Andrea Desiderato (AD)